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Journal Article

Application of TCA cycle metabolism for growth estimates of heterotrophic bacterioplankton

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Overbeck,  Hans-Jürgen
Department Microbial Ecology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Overbeck, H.-J. (1984). Application of TCA cycle metabolism for growth estimates of heterotrophic bacterioplankton. Ergebnisse der Limnologie/Advances in Limnology, 19, 23-36.


Cite as: https://hdl.handle.net/21.11116/0000-0005-58B5-C
Abstract
CO2 dark fixation is a basic metabolic process, related with the tricarboxylic acid (TCA)
cycle and biosynthetic processes, starting from the pool of TCA cycle intermediates. In bacteria
phospho-enolpyruvate-carboxylase (PEP-carboxylase) is one of the key enzymes for carboxylation of C3 -compounds under formation of oxaloacetate. The fine regulation of PEP-carboxylase is rather complex. TCA cycle intermediates can be inhibitors. The CO2 dark uptake reflects
the metabolic activity of the bacterial cell: only in the presence of degradable substrates 14-CO2
is taken up. The rate of ta CO2 uptake depends on the amount of available substrates. Growth
estimates derived from 14-CO2 -uptake and independent direct growth estimates with a digitizer-
microcomputer system on scanning electron micrographs agree rather well.