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Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish

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Thierbach,  Konstantin
Institute for Medical Informatics and Biometry, University Hospital Carl Gustav Carus, Dresden, Germany;
Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society;

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Waschke,  Johannes
Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society;
Faculty of Computer Science and Media, University of Applied Sciences, Germany;

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Scherf,  Nico
Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society;

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Shah_2019.pdf
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Citation

Shah, G., Thierbach, K., Schmid, B., Waschke, J., Reade, A., Hlawitschka, M., et al. (2019). Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish. Nature Communications, 10: 5753. doi:10.1038/s41467-019-13625-0.


Cite as: http://hdl.handle.net/21.11116/0000-0005-638F-B
Abstract
The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement characteristics that are predominantly determined by their position in the embryo. The differences in initial germlayer distributions are subsequently amplified by a global movement, which organizes the organ precursors along the embryonic body axis, giving rise to the blueprint of organ formation. The tools and data are available as a resource for the community.