Bottom-up synthetic biology: reconstitution in space and time

Jia, Haiyang; Schwille, Petra

doi:10.1016/j.copbio.2019.05.008

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Bottom-up synthetic biology: reconstitution in space and time

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Jia, Haiyang
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Schwille, Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Jia, H., & Schwille, P. (2019). Bottom-up synthetic biology: reconstitution in space and time. Current Opinion in Biotechnology, 60, 179-187. doi:10.1016/j.copbio.2019.05.008.

Cite as: https://hdl.handle.net/21.11116/0000-0005-A073-4

Abstract

Bottom-up reconstituting well-characterized functional molecular entities, parts and modules towards a synthetic cell will give new insights into the mechanisms and origin of life. However, a remaining central challenge is how to organize cellular processes spatiotemporally from their component parts in vitro. Here, we review cutting edge tools and technologies that can facilitate such a bottom-up reconstitution towards a synthetic cell in space and time, particularly with regard to the following aspects: (1) reliable model membrane-environment and microenvironment; (2) dynamic genetic regulation and self-sustaining transcription and translation machinery; (3) spatially organized cytoskeleton that supports the biological architecture and cellular self-reproduction in 3D.