Bioluminescent imaging of single bacterial cells using an enhanced ilux operon.

Gregor, C.

doi:10.1007/978-1-4939-9940-8_4

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Bioluminescent imaging of single bacterial cells using an enhanced ilux operon.

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Gregor, C.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Gregor, C. (2020). Bioluminescent imaging of single bacterial cells using an enhanced ilux operon. In S. Ripp (Ed.), Bioluminescent Imaging. New York: Humana Pr. doi:10.1007/978-1-4939-9940-8_4.

Cite as: https://hdl.handle.net/21.11116/0000-0005-6F09-6

Abstract

The lux operon is a useful reporter for bioluminescence imaging due to its independence of exogenous luciferin supply, but its relatively low brightness hampers the imaging of single cells. This chapter describes a procedure for the imaging of individual Escherichia coli cells using an improved ilux operon. The enhanced brightness of ilux enables long-term bioluminescence imaging of single bacteria with high sensitivity without the requirement for an external luciferin.