English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Low-signal limit of X-ray single particle diffractive imaging

MPS-Authors

Ayyer,  K.
Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron DESY;

/persons/resource/persons194656

Paulraj,  L. X.
International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron DESY;
Linac Coherent Light Source, SLAC National Accelerator Laboratory;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

oe-27-26-37816.pdf
(Publisher version), 961KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Ayyer, K., Morgan, A. J., Aquila, A., DeMirci, H., Hogue, B. G., Kirian, R. A., et al. (2019). Low-signal limit of X-ray single particle diffractive imaging. Optics Express, 27(26), 37816-37833. doi:10.1364/OE.27.037816.


Cite as: https://hdl.handle.net/21.11116/0000-0005-7022-6
Abstract
An outstanding question in X-ray single particle imaging experiments has been the feasibility of imaging sub 10-nm-sized biomolecules under realistic experimental conditions where very few photons are expected to be measured in a single snapshot and instrument background may be significant relative to particle scattering. While analyses of simulated data have shown that the determination of an average image should be feasible using Bayesian methods such as the EMC algorithm, this has yet to be demonstrated using experimental data containing realistic non-isotropic instrument background, sample variability and other experimental factors. In this work, we show that the orientation and phase retrieval steps work at photon counts diluted to the signal levels one expects from smaller molecules or with weaker pulses, using data from experimental measurements of 60-nm PR772 viruses. Even when the signal is reduced to a fraction as little as 1/256, the virus electron density determined using ab initio phasing is of almost the same quality as the high-signal data. However, we are still limited by the total number of patterns collected, which may soon be mitigated by the advent of high repetition-rate sources like the European XFEL and LCLS-II.