English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

The exonuclease Xrn1 activates transcription and translation of mRNAs encoding membrane proteins

MPS-Authors
/persons/resource/persons239343

Nedialkova,  Danny D.
Nedialkova, Danny / Mechanisms of Protein Biogenesis, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

s41467-019-09199-6.pdf
(Any fulltext), 3MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Blasco-Moreno, B., de Campos-Mata, L., Bottcher, R., Garcia-Martinez, J., Jungfleisch, J., Nedialkova, D. D., et al. (2019). The exonuclease Xrn1 activates transcription and translation of mRNAs encoding membrane proteins. NATURE COMMUNICATIONS, 10: 1298. doi:10.1038/s41467-019-09199-6.


Cite as: https://hdl.handle.net/21.11116/0000-0005-8F0B-F
Abstract
The highly conserved 5'-3' exonuclease Xrn1 regulates gene expression in eukaryotes by coupling nuclear DNA transcription to cytosolic mRNA decay. By integrating transcriptome-wide analyses of translation with biochemical and functional studies, we demonstrate an unanticipated regulatory role of Xrn1 in protein synthesis. Xrn1 promotes translation of a specific group of transcripts encoding membrane proteins. Xrnl-dependence for translation is linked to poor structural RNA contexts for translation initiation, is mediated by interactions with components of the translation initiation machinery and correlates with an Xrnl-dependence for mRNA localization at the endoplasmic reticulum, the translation compartment of membrane proteins. Importantly, for this group of mRNAs, Xrn1 stimulates transcription, mRNA translation and decay. Our results uncover a crosstalk between the three major stages of gene expression coordinated by Xrn1 to maintain appropriate levels of membrane proteins.