Cryogenic localization of single molecules with angstrom precision

Weisenburger, Siegfried; Bo, Jing; Renn, Alois; Sandoghdar, Vahid

doi:10.1117/12.2025373

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Cryogenic localization of single molecules with angstrom precision

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Weisenburger, Siegfried
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Sandoghdar, Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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引用

Weisenburger, S., Bo, J., Renn, A., & Sandoghdar, V. (2013). Cryogenic localization of single molecules with angstrom precision. Proceedings of SPIE, 8815:. doi:10.1117/12.2025373.

引用: https://hdl.handle.net/21.11116/0000-0005-8A7D-4

要旨

The precision in localizing a molecule is ultimately determined by the number of detected photons, which is in turn limited by photobleaching. Currently, fluorophores can be routinely localized to a few tens of nanometers at room temperature. In this work we demonstrate localization precision better than 3 Angstrom by substantial improvement of the molecular photostability at cryogenic temperatures. We discuss the challenges, solutions and promise of our methodology for high-performance co-localization and super-resolution microscopy.