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Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

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Wisniewski,  Jacek R.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Zettl,  Katharina
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Wisniewski, J. R., & Zettl, K. (2019). Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation. DATA IN BRIEF, 26: 104530. doi:10.1016/j.dib.2019.104530.


Cite as: http://hdl.handle.net/21.11116/0000-0005-9213-0
Abstract
Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wisniewski, 2019). (C) 2019 The Authors. Published by Elsevier Inc.