Presence of a 1,25-dihydroxy-vitamin D3 receptor in chick skeletal muscle 
myoblasts

Boland, Ricardo; Norman, Anthony; Ritz, Eberhard; Hasselbach, Wilhelm

doi:10.1016/0006-291X(85)91679-1

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Presence of a 1,25-dihydroxy-vitamin D3 receptor in chick skeletal muscle myoblasts

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Hasselbach, Wilhelm
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/0006291X85916791/pdf?md5=08f0280168d26beb00e443d536cc3f57&pid=1-s2.0-0006291X85916791-main.pdf
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Citation

Boland, R., Norman, A., Ritz, E., & Hasselbach, W. (1985). Presence of a 1,25-dihydroxy-vitamin D3 receptor in chick skeletal muscle myoblasts. Biochemical and Biophysical Research Communications, 128(1), 305-311. doi:10.1016/0006-291X(85)91679-1.

Cite as: https://hdl.handle.net/21.11116/0000-0005-9226-B

Abstract

The presence of a specific receptor for 1,25-dihydroxy-vitamin D3 was investigated in myoblasts released from chick embryo skeletal muscle by trypsin and collagenase treatment. Density gradient analysis of the cytosol obtained from these muscle cell preparations showed that 1,25-dihydroxy-vitamin D3 binds specifically to a 3.7 S macromolecule. Scatchard analysis yielded an equilibrium dissociation constant of 2.46 x 10(-10) M and a Nmax of 74 fmol/mg of cytosol protein. The data is in agreement with previous evidence which indicates that the action of the vitamin D metabolite on muscle Ca uptake is mediated by de novo protein and RNA synthesis, and supports the concept that muscle is a target organ for 1,25-dihydroxy-vitamin D3.