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Journal Article

A tunable refractive index matching medium for live imaging cells, tissues and model organisms.

MPS-Authors
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Boothe,  T.
Department of Tissue Dynamics and Regeneration, MPI for Biophysical Chemistry, Max Planck Society;

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Rink,  J. C.
Department of Tissue Dynamics and Regeneration, MPI for Biophysical Chemistry, Max Planck Society;

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3190223.pdf
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3190223_Suppl.htm
(Supplementary material), 180KB

Citation

Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W. B., Zaburdaev, V., et al. (2017). A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLife, 6: e27240. doi:10.7554/eLife.27240.


Cite as: https://hdl.handle.net/21.11116/0000-0005-924E-F
Abstract
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.