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Journal Article

Imidazole-imidazole hydrogen bonding in the pH-sensing histidine side chains of influenza A M2.

MPS-Authors
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Tekwani Movellan,  K.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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Wegstroth,  M.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Overkamp,  K.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Leonov,  A.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Becker,  S.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Andreas,  L. B.
Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society;

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Fulltext (public)

3190403.pdf
(Publisher version), 2MB

Supplementary Material (public)

3190403_Suppl.pdf
(Supplementary material), 523KB

Citation

Tekwani Movellan, K., Wegstroth, M., Overkamp, K., Leonov, A., Becker, S., & Andreas, L. B. (2020). Imidazole-imidazole hydrogen bonding in the pH-sensing histidine side chains of influenza A M2. Journal of the American Chemical Society, 142(6), 2704-2708. doi:10.1021/jacs.9b10984.


Cite as: https://hdl.handle.net/21.11116/0000-0005-93D2-7
Abstract
The arrangement of histidine side chains in influenza A M2 tetramer determines their pKa values, which define pH-controlled proton conduction critical to the virus lifecycle. Both water-associated and hydrogen-bonded imidazole-imidazolium histidine quaternary structures have been proposed, based on crystal structures and NMR chemical shifts, respectively. Here we show, using the conduction domain construct of M2 in lipid bilayers, that the imidazole rings are hydrogen bonded even at a pH of 7.8 in the neutral charge state. An intermolecular 8.9 ± 0.3 Hz 2hJNN hydrogen bond is observed between H37 Nε and Nδ recorded in a fully protonated sample with 100 kHz magic-angle spinning. This interaction could not be detected in the drug-bound sample.