Expanding the genome-targeting scope and the site selectivity of high-precision 
base editors

Tan, J.; Zhang, F.; Karcher, D.; Bock, R.

doi:10.1038/s41467-020-14465-z

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Expanding the genome-targeting scope and the site selectivity of high-precision base editors

MPS-Authors

/persons/resource/persons229809

Tan, J.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons229811

Zhang, F.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97218

Karcher, D.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97077

Bock, R.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Tan, J., Zhang, F., Karcher, D., & Bock, R. (2020). Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nature Communications, 11(1): 629. doi:10.1038/s41467-020-14465-z.

Cite as: https://hdl.handle.net/21.11116/0000-0005-9516-A

Abstract

Base editors (BEs) are RNA-guided CRISPR-Cas-derived genome editing tools that induce single-nucleotide changes. The limitations of current BEs lie in their low precision (especially when multiple target nucleotides of the deaminase are present within the activity window) and their restriction to targets that are in proper distance from the PAM sequence. We have recently developed high-precision cytidine BEs by engineering CDA1 truncations and nCas9 fusions that predominantly edit nucleotide C−18 relative to the PAM sequence NGG. Here, by testing fusions with Cas9 variants that recognize alternative PAMs, we provide a series of high-precision BEs that greatly expand the versatility of base editing. In addition, we obtained BEs that selectively edit C−15 or C−16. We also show that our high-precision BEs can substantially reduce off-target effect. These improved base editing tools will be widely applicable in basic research, biotechnology and gene therapy.