Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Monosomes actively translate synaptic mRNAs in neuronal processes


Langer,  Julian David
Proteomics and Mass Spectrometry, Max Planck Institute of Biophysics, Max Planck Society;
Max Planck Institute for Brain Research, Frankfurt, Germany;

External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Biever, A., Glock, C., Tushev, G., Ciirdaeva, E., Dalmay, T., Langer, J. D., et al. (2020). Monosomes actively translate synaptic mRNAs in neuronal processes. Science, 367(6477): eaay4991, pp. 526. doi:10.1126/science.aay4991.

Cite as: http://hdl.handle.net/21.11116/0000-0005-9558-0
To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome.