Mu opioid receptor mRNA expression in SHSY-5Y human neuroblastoma cells

Yu, X; Blake, AD; Mao, X; Chang, SL

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Mu opioid receptor mRNA expression in SHSY-5Y human neuroblastoma cells

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Yu, X., Blake, A., Mao, X., & Chang, S. (2002). Mu opioid receptor mRNA expression in SHSY-5Y human neuroblastoma cells. Poster presented at 32nd Annual Meeting of the Society for Neuroscience (Neuroscience 2002), Orlando, FL, USA.

Cite as: https://hdl.handle.net/21.11116/0000-0005-961A-5

Abstract

Prolonged exposure to morphine down-regulates mu opioid receptors (MOR) on both undifferentiated and differentiated (retinoic acid or phorbol ester treated) SHSY-5Y cells. However, morphine pretreatment does not alter MOR receptor affinity for morphine. To investigate the molecular basis for morphine’s regulatory effects, we have developed a quantitative competitive reverse transcriptase-polymerase chain reaction (QC-RT-PCR) to quantify the amount of MOR present in SHSY-5Y cells. SHSY-5Y cells were differentiated and then incubated for 24 h with vehicle, morphine, naloxone, or both morphine and naloxone. Expression of MOR mRNA in undifferentiated SHSY-5Y cells was determined to be 369 fmol MOR mRNA/mg total RNA. Differentiation of SHSY-5Y cells with retinoic acid or phorbol ester up-regulated MOR mRNA expression by 40 \% and 78\%, respectively. A 24 hour treatment with morphine (10 mM) down-regulated MOR mRNA, an effect that was partially reversed by naloxone. Naloxone treatment alone upregulated MOR mRNA in undifferentiated, but not in RA-differentiated SHSY-5Y cells. Taken together, a significant component of SHSY-5Y cellular adaptation during chronic morphine treatment may occur at the mRNA level.