H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in 
chloroplast ATP synthase mutants

Correa Galvis, V.; Strand, D.; Messer, M.; Thiele, W.; Bethmann, Stephanie; Hübner, D.; Uflewski, M.; Kaiser, E.; Siemiatkowska, B.; Morris, B. A.; Toth, Szilvia Z.; Watanabe, M.; Brückner, F.; Hoefgen, R.; Jahns, Peter; Schöttler, M. A.; Armbruster, U.

doi:10.1104/pp.19.01561

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学術論文

H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants

MPS-Authors

/persons/resource/persons203729

Correa Galvis, V.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons198698

Strand, D.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons239894

Messer, M.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97438

Thiele, W.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons245228

Hübner, D.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons245230

Uflewski, M.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons231593

Kaiser, E.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons231425

Siemiatkowska, B.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons245232

Morris, B. A.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97470

Watanabe, M.
Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons217680

Brückner, F.
Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97197

Hoefgen, R.
Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97392

Schöttler, M. A.
Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons183454

Armbruster, U.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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引用

Correa Galvis, V., Strand, D., Messer, M., Thiele, W., Bethmann, S., Hübner, D., Uflewski, M., Kaiser, E., Siemiatkowska, B., Morris, B. A., Toth, S. Z., Watanabe, M., Brückner, F., Hoefgen, R., Jahns, P., Schöttler, M. A., & Armbruster, U. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiology, 182, 2126-2142. doi:10.1104/pp.19.01561.

引用: https://hdl.handle.net/21.11116/0000-0006-0FF4-7

要旨

The composition of the thylakoid proton motive force (pmf) is regulated by thylakoid ion transport. Passive ion channels in the thylakoid membrane dissipate the membrane potential (Δψ) component to allow for a higher fraction of pmf stored as a proton concentration gradient (ΔpH). K+/H+ antiport across the thylakoid membrane via K+ EXCHANGE ANTIPORTER3 (KEA3) instead reduces the ΔpH fraction of the pmf. Thereby, KEA3 decreases non-photochemical quenching (NPQ), thus allowing for higher light use efficiency, which is particularly important during transitions from high to low light. Here, we show that in the background of the Arabidopsis thaliana chloroplast (cp)ATP synthase assembly mutant cgl160, with decreased cpATP synthase activity and increased pmf amplitude, KEA3 plays an important role for photosynthesis and plant growth under steady state conditions. By comparing cgl160 single with cgl160 kea3 double mutants, we demonstrate that in the cgl160 background loss of KEA3 causes a strong growth penalty. This is due to a decreased photosynthetic capacity of cgl160 kea3 mutants, as these plants have a lower lumenal pH than cgl160 mutants, and thus show substantially increased pH-dependent NPQ and decreased electron transport through the cytochrome b6f complex. Overexpression of KEA3 in the cgl160 background decreases pH-dependent NPQ and increases photosystem II efficiency. Taken together, our data provide evidence that under conditions where cpATP synthase activity is low, a KEA3-dependent reduction of ΔpH benefits photosynthesis and growth.