DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea.

Yin, Xiuran; Kulkarni, Ajinkya C; Friedrich, Michael W

doi:10.1007/978-1-4939-9721-3_15

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DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea.

MPS-Authors

Yin, Xiuran
IMPRS MarMic, Max Planck Institute for Marine Microbiology, Max Planck Society;

Kulkarni, Ajinkya C
IMPRS MarMic, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Yin, X., Kulkarni, A. C., & Friedrich, M. W. (2019). DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea. Methods in Molecular Biology, 2046, 189-206. doi:10.1007/978-1-4939-9721-3_15.

Cite as: https://hdl.handle.net/21.11116/0000-0005-BA20-5

Abstract

Methylotrophic methanogenic archaea are an integral part of the carbon
cycle in various anaerobic environments. Different from methylotrophic
bacteria, methylotrophic methanogens assimilate both, the methyl
compound and dissolved inorganic carbon. Here, we present DNA- and
RNA-stable isotope probing (SIP) methods involving an effective labeling
strategy using 13C-labeled dissolved inorganic carbon (DIC) as carbon
source along with methanol as dissimilatory substrate.