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High-resolution 3D light microscopy with STED and RESOLFT

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Sahl,  S. J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Sahl, S. J., & Hell, S. W. (2019). High-resolution 3D light microscopy with STED and RESOLFT. In J. F. Bille (Ed.), High resolution imaging in microscopy and ophthalmology (pp. 3-32). Cham: Springer. doi:10.1007/978-3-030-16638-0_1.


Cite as: http://hdl.handle.net/21.11116/0000-0005-BE27-A
Abstract
This chapter discusses the simple yet powerful ideas which have allowed to break the diffraction resolution limit of lens-based optical microscopy. The basic principles and standard implementations of STED (stimulated emission depletion) and RESOLFT (reversible saturable/switchable optical linear (fluorescence) transitions) microscopy are introduced, followed by selected highlights of recent advances, including MINFLUX (minimal photon fluxes) nanoscopy with molecule-size (~1 nm) resolution.