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Journal Article

The HARE chip for efficient time-resolved serial synchrotron crystallography

MPS-Authors
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Mehrabi,  P.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Müller-Werkmeister,  H.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Institute of Chemistry – Physical Chemistry, University of Potsdam;

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Leimkohl,  J.-P.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Schikora,  H.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Epp,  S. W.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Tellkamp,  F.
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Schulz,  E.-C.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Miller,  R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Department of Physics, Universität Hamburg;
Departments of Chemistry and Physics, University of Toronto;

External Ressource
Fulltext (public)

wz5004.pdf
(Publisher version), 2MB

Supplementary Material (public)

suppl.zip
(Supplementary material), 6MB

Citation

Mehrabi, P., Müller-Werkmeister, H., Leimkohl, J.-P., Schikora, H., Ninkovic, J., Krivokuca, S., et al. (2020). The HARE chip for efficient time-resolved serial synchrotron crystallography. Journal of Synchrotron Radiation; Wiley-Blackwell on behalf of the IUCr, 27(2), 360-370. doi:10.1107/S1600577520000685.


Cite as: http://hdl.handle.net/21.11116/0000-0005-C0EC-8
Abstract
Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.