English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

The influence of metal ion complexation on 31P-NMR parameters of phosphorothioates of guanosine nucleotides

MPS-Authors
/persons/resource/persons95056

Rösch,  Paul
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons98693

Goody,  Roger S.
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

/persons/resource/persons211012

Isakov,  Maria
Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.tandfonline.com/doi/abs/10.1080/03086648408073121#aHR0cHM6Ly93d3cudGFuZGZvbmxpbmUuY29tL2RvaS9wZGYvMTAuMTA4MC8wMzA4NjY0ODQwODA3MzEyMUBAQDA=
(Any fulltext)

https://doi.org/10.1080/03086648408073121
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Rösch, P., Goody, R. S., & Isakov, M. (1984). The influence of metal ion complexation on 31P-NMR parameters of phosphorothioates of guanosine nucleotides. Phosphorus Sulfur and Silicon and the Related Elements, 9-16. doi:10.1080/03086648408073121.


Cite as: https://hdl.handle.net/21.11116/0000-0005-DFBB-E
Abstract
An extension of methods already used for the preparation of adenosine phosphorothioates was used to prepare guanosine phosphorothioates in quantities sufficient for extensive 31P-NMR studies. Guanosine phosphorothioates were used to exemplify the influence of Cd2+ and Mg2+ complexation on 31P-NMR parameters as pK-values, chemical shifts, and P[sbnd]O[sbnd]P coupling constants. pKa values decrease in general in the order pK(α-S) a > pK(β-S) a > pK(γ-S) a; the magnitude of the pKa decrease on metal complexation is also in this order. The chemical shifts for the P[sbnd]S resonances are changed downfield on Mg2+ complexation and upfield on Cd2+ complexation. P[sbnd]O[sbnd]P coupling constants are increased by about 7 Hz on sulfur substitution at one of the contributing phosphorus atoms; this difference is at least partially ineffective in the Cd2+ complexes.