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Conference Paper

The Deoxyglucose Method for Insects: towards Electron Microscopical Resolution

MPS-Authors
/persons/resource/persons246234

Buchner,  E
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons246243

Buchner,  S
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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https://www.karger.com/Article/Abstract/115224
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Citation

Buchner, E., & Buchner, S. (1981). The Deoxyglucose Method for Insects: towards Electron Microscopical Resolution. European Neurology, 20(3), 152-156.


Cite as: https://hdl.handle.net/21.11116/0000-0005-E8D0-A
Abstract
The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying may turn out to be the best method of preparing the tissue for plastic embedding. The problem of cutting on a water surface is discussed. It is demonstrated that freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.