Cytidine deaminase 2 is required for VLRB antibody gene assembly in lampreys

Morimoto,  Ryo; O’Meara, Connor P.; Holland, Stephen J.; Trancoso, I.; Souissi, Ahmed; Schorpp, Michael; Vassaux, Danièle; Iwanami, Norimasa; Giorgetti, Orlando Bruno; Evanno, Guillaume; Boehm, Thomas

doi:10.1126/sciimmunol.aba0925

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Cytidine deaminase 2 is required for VLRB antibody gene assembly in lampreys

MPS-Authors

Morimoto, Ryo
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

O’Meara, Connor P.
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Holland, Stephen J.
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Trancoso, I.
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Schorpp, Michael
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Iwanami, Norimasa
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Giorgetti, Orlando Bruno
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Boehm, Thomas
Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

External Resource

https://immunology.sciencemag.org/content/5/45/eaba0925.long
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Morimoto et al..pdf
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Citation

Morimoto, R., O’Meara, C. P., Holland, S. J., Trancoso, I., Souissi, A., Schorpp, M., et al. (2020). Cytidine deaminase 2 is required for VLRB antibody gene assembly in lampreys. Science Magazine, 5, eaba0925. doi:10.1126/sciimmunol.aba0925.

Cite as: http://hdl.handle.net/21.11116/0000-0008-0AA0-8

Abstract

The antibodies of jawless vertebrates consist of leucine-rich repeat arrays encoded by somatically assembled VLRB genes. It is unknown how the incomplete germline VLRB loci are converted into functional antibody genes during B lymphocyte development in lampreys. In Lampetra planeri larvae lacking the cytidine deaminase CDA2 gene, VLRB assembly fails, whereas the T lineage-associated VLRA and VLRC antigen receptor gene assemblies occur normally. Thus, CDA2 acts in a B cell lineage-specific fashion to support the somatic diversification of VLRB antibody genes. CDA2 is closely related to activation-induced cytidine deaminase (AID), which is essential for the elaboration of immunoglobulin gene repertoires in jawed vertebrates. Our results thus identify a convergent mechanism of antigen receptor gene assembly and diversification that independently evolved in the two sister branches of vertebrates.