Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

Löser, Eva; Latrielle, Daniel; Iovino, Nicola

doi:10.1007/978-1-4939-6380-5_28

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Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos

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Löser, Eva
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Latrielle, Daniel
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Iovino, Nicola
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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https://link.springer.com/protocol/10.1007%2F978-1-4939-6380-5_3
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Citation

Löser, E., Latrielle, D., & Iovino, N. (2016). Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos. In Methods in Molecular Biology (pp. 23-36).

Cite as: https://hdl.handle.net/21.11116/0000-0006-054E-E

Abstract

This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq).