Analysis of biomechanical properties of hematopoietic stem and progenitor cells 
with Real-Time Deformability Cytometry

Jacobi, Angela; Rosendahl, Philipp; Kräter, Martin; Urbanska, Marta; Herbig, Maik; Guck, Jochen

doi:10.1007/978-1-4939-9574-5_11

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Analysis of biomechanical properties of hematopoietic stem and progenitor cells with Real-Time Deformability Cytometry

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Guck, Jochen
Biotechnology Center, Technische Universität Dresden, Germany;
Guck Division, Max Planck Institute for the Science of Light, Max Planck Society;
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

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Citation

Jacobi, A., Rosendahl, P., Kräter, M., Urbanska, M., Herbig, M., & Guck, J. (2019). Analysis of biomechanical properties of hematopoietic stem and progenitor cells with Real-Time Deformability Cytometry. In Methods in Molecular Biology (pp. 135-148). US: Springer. doi:10.1007/978-1-4939-9574-5_11.

Cite as: https://hdl.handle.net/21.11116/0000-0006-0A55-0

Abstract

Stem cell mechanics, determined predominantly by the cell's cytoskeleton, plays an important role in different biological processes such as stem cell differentiation or migration. Several methods to measure mechanical properties of cells are currently available, but most of them are limited in the ability to screen large heterogeneous populations in a robust and efficient manner-a feature required for successful translational applications. With real-time fluorescence and deformability cytometry (RT-FDC), mechanical properties of cells in suspension can be screened continuously at rates of up to 1,000 cells/s-similar to conventional flow cytometers-which makes it a suitable method not only for basic research but also for a clinical setting. In parallel to mechanical characterization, RT-FDC allows to measure specific molecular markers using standard fluorescence labeling. In this chapter, we provide a detailed protocol for the characterization of hematopoietic stem and progenitor cells (HSPCs) in heterogeneous mobilized peripheral blood using RT-FDC and present a specific morpho-rheological fingerprint of HSPCs that allows to distinguish them from all other blood cell types.