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Journal Article

Monosomes actively translate synaptic mRNAs in neuronal processes

MPS-Authors

Biever,  Anne
Max Planck Institute of Biophysics, Max Planck Society;

Glock,  Caspar
Max Planck Institute of Biophysics, Max Planck Society;

Tushev,  Georgi
Max Planck Institute of Biophysics, Max Planck Society;

Ciirdaeva,  Elena
Max Planck Institute of Biophysics, Max Planck Society;

Dalmay,  Tushev
Max Planck Institute of Biophysics, Max Planck Society;

Langer,  Julian D.
Max Planck Institute of Biophysics, Max Planck Society;
Max Planck Institute for Brain Research, Max Planck Society;

Schuman,  Erin M.
Max Planck Institute of Biophysics, Max Planck Society;
Max Planck Institute for Brain Research, Frankfurt, Germany;

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Citation

Biever, A., Glock, C., Tushev, G., Ciirdaeva, E., Dalmay, T., Langer, J. D., et al. (2020). Monosomes actively translate synaptic mRNAs in neuronal processes. Science, 367(6477). doi:10.1126/science.aay4991.


Cite as: http://hdl.handle.net/21.11116/0000-0006-0931-9
Abstract
To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome.