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Journal Article

Optical recording with single cell resolution from monolayered slice cultures of rat hippocampus

MPS-Authors
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Bonhoeffer,  T
Former Department Structure and Function of Natural Nerve-Net , Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Staiger,  V
Former Department Structure and Function of Natural Nerve-Net , Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/030439408890599X
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Citation

Bonhoeffer, T., & Staiger, V. (1988). Optical recording with single cell resolution from monolayered slice cultures of rat hippocampus. Neuroscience Letters, 92(3), 259-264. doi:10.1016/0304-3940(88)90599-x.


Cite as: https://hdl.handle.net/21.11116/0000-0006-0C3E-9
Abstract
Voltage-sensitive dyes were used to optically record the membrane potentials from neurons in hippocampal slice cultures. Multi-channel recordings from these monolayered but otherwise 'organotypic' slice cultures had very good spatial as well as good temporal resolution (15 x 15 micron, 0.5 ms respectively). We show that in this preparation action potentials elicited by intracellular current pulses can be recorded optically and that single spikes are readily detectable without averaging. Furthermore, a new procedure which significantly reduces photodynamic damage is described. Our study demonstrates the feasibility of optical recording with single cell resolution in an organotypic mammalian CNS preparation.