User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse




Journal Article

In vitro analysis of chemotactic leukocyte migration in 3D environments


Lämmermann,  Tim
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

External Ressource
Fulltext (public)

Sixt et al..pdf
(Publisher version), 464KB

Supplementary Material (public)
There is no public supplementary material available

Sixt, M., & Lämmermann, T. (2011). In vitro analysis of chemotactic leukocyte migration in 3D environments. Methods in Molecular Biology, 32, 703-713. doi:10.1007/978-1-61779-207-6_11.

Cite as: http://hdl.handle.net/21.11116/0000-0006-0C91-9
Cell migration on two-dimensional (2D) substrates follows entirely different rules than cell migration in three-dimensional (3D) environments. This is especially relevant for leukocytes that are able to migrate in the absence of adhesion receptors within the confined geometry of artificial 3D extracellular matrix scaffolds and within the interstitial space in vivo. Here, we describe in detail a simple and economical protocol to visualize dendritic cell migration in 3D collagen scaffolds along chemotactic gradients. This method can be adapted to other cell types and may serve as a physiologically relevant paradigm for the directed locomotion of most amoeboid cells.