In vitro analysis of chemotactic leukocyte migration in 3D environments

Sixt, Michael; Lämmermann, Tim

doi:10.1007/978-1-61779-207-6_11

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In vitro analysis of chemotactic leukocyte migration in 3D environments

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Lämmermann, Tim
Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pubmed/21748675
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Citation

Sixt, M., & Lämmermann, T. (2011). In vitro analysis of chemotactic leukocyte migration in 3D environments. Methods in Molecular Biology, 32, 703-713. doi:10.1007/978-1-61779-207-6_11.

Cite as: https://hdl.handle.net/21.11116/0000-0006-0C91-9

Abstract

Cell migration on two-dimensional (2D) substrates follows entirely different rules than cell migration in three-dimensional (3D) environments. This is especially relevant for leukocytes that are able to migrate in the absence of adhesion receptors within the confined geometry of artificial 3D extracellular matrix scaffolds and within the interstitial space in vivo. Here, we describe in detail a simple and economical protocol to visualize dendritic cell migration in 3D collagen scaffolds along chemotactic gradients. This method can be adapted to other cell types and may serve as a physiologically relevant paradigm for the directed locomotion of most amoeboid cells.