Serial optical coherence microscopy for label-free volumetric histopathology

Min, E; Ban, S; Lee, J; Vavilin, A; Baek, S; Jung, S; Ahn, Y; Park, K; Shin, S; Han, S; Cho, H; Lee-Kwon, W; Kim, J; Lee, CJ; Jung, W

doi:10.1038/s41598-020-63460-3

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Serial optical coherence microscopy for label-free volumetric histopathology

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Min, E
Research Group Systems Neuroscience & Neuroengineering, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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https://www.nature.com/articles/s41598-020-63460-3.pdf
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Citation

Min, E., Ban, S., Lee, J., Vavilin, A., Baek, S., Jung, S., et al. (2020). Serial optical coherence microscopy for label-free volumetric histopathology. Scientific Reports, 10(1): 6711, pp. 1-8. doi:10.1038/s41598-020-63460-3.

Cite as: https://hdl.handle.net/21.11116/0000-0006-4377-9

Abstract

The observation of histopathology using optical microscope is an essential procedure for examination of tissue biopsies or surgically excised specimens in biological and clinical laboratories. However, slide-based microscopic pathology is not suitable for visualizing the large-scale tissue and native 3D organ structure due to its sampling limitation and shallow imaging depth. Here, we demonstrate serial optical coherence microscopy (SOCM) technique that offers label-free, high-throughput, and large-volume imaging of ex vivo mouse organs. A 3D histopathology of whole mouse brain and kidney including blood vessel structure is reconstructed by deep tissue optical imaging in serial sectioning techniques. Our results demonstrate that SOCM has unique advantages as it can visualize both native 3D structures and quantitative regional volume without introduction of any contrast agents.