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Journal Article

Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

MPS-Authors
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Klioutchnikov,  Alexandr
Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Wallace,  Damian J       
Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Sawinski,  Jürgen       
Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Pawlak,  Verena
Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Voit,  Kay-Michael
Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Kerr,  Jason N. D.       
Department of Behavior and Brain Organization, Center of Advanced European Studies and Research (caesar), Max Planck Society;

External Resource

https://www.nature.com/articles/s41592-020-0817-9#Abs1
(Abstract)

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Supplementary Material (public)

Zemax_archive_files_for_custom_optics.zip
(Supplementary material), 297KB

Citation

Klioutchnikov, A., Wallace, D. J., Frosz, M. H., Zeltner, R., Sawinski, J., Pawlak, V., et al. (2020). Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats. Nature methods, 17, 509-513. doi:10.1038/s41592-020-0817-9.


Cite as: https://hdl.handle.net/21.11116/0000-0006-5153-1
Abstract
We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 euronal activity for >1 h in freely moving rats performing a range of behaviors.