English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

What's the catch?: Archaeological application of rapid collagen-based species identification for Pacific Salmon

MPS-Authors
/persons/resource/persons72602

Britton,  Kate
Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Richter, K. K., McGrathan, K., MassonMacLeanc, E., Hickinbotham, S., Tedder, A., Britton, K., et al. (2020). What's the catch?: Archaeological application of rapid collagen-based species identification for Pacific Salmon. Journal of Archaeological Science: Reports, 116: 105116. doi:10.1016/j.jas.2020.105116.


Cite as: http://hdl.handle.net/21.11116/0000-0006-5649-8
Abstract
Pacific salmon (Oncorhynchus spp.) are ecological and cultural keystone species along the Northwest Coast of North America and are ubiquitous in archaeological sites of the region. The inability to morphologically identify salmonid post-cranial remains to species, however, can limit our understanding of the ecological and cultural role different taxa played in the seasonal subsistence practices of Indigenous groups in the past. Here, we present a rapid, cost-effective ZooMS method to distinguish salmonid species based on collagen peptide mass-fingerprinting. Using modern reference material and an assemblage of 28 DNA-identified salmonid bones from the pre-contact Yup'ik site of Nunalleq, Western Alaska, we apply high-resolution mass spectrometry (LC-MS/MS) to identify a series of potential collagen peptide markers to distinguish Pacific salmon. We then confirm these peptide markers with a blind ZooMS analysis (MALDI-TOF-MS) of the archaeological remains. We successfully distinguish five species of anadromous salmon with this ZooMS approach, including one specimen that could not be identified through ancient DNA analysis. Our biomolecular identification of chum (43), sockeye (21), chinook (18), coho (11) and pink (7), confirm the exploitation of all five available species of salmonid at Nunalleq. © 2020 Elsevier Ltd