English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Direct determination of phospholipid lamellar structure at 0.34-nm resolution

MPS-Authors
/persons/resource/persons21343

Beckmann,  Erich
Electron Microscopy, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons22285

Zemlin,  Friedrich
Electron Microscopy, Fritz Haber Institute, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Dorset, D. L., Beckmann, E., & Zemlin, F. (1990). Direct determination of phospholipid lamellar structure at 0.34-nm resolution. Proceedings of the National Academy of Sciences of the United States of America, 87(19), 7570-7573. doi:10.1073/pnas.87.19.7570.


Cite as: https://hdl.handle.net/21.11116/0000-0006-64B4-E
Abstract
Low-dose, high-resolution electron microscopy combined with conventional direct-phasing methods
based on the estimates of triplet-structure invariants are used
to determine phase values for all observed electron-diffractionstructure factor magnitudes from epitaxially oriented multilamellar paracrystals of the phosphospholipid 1,2-dihexadecylsn-glycerophosphoethanolamine. The reverse Fourier transform of these phased-structure factors is a one-dimensional
electrostatic potential map that strongly resembles the electrondensito maps calculated from similar x-ray-diffraction data.
Determination of the phase values for the electron-diffraction
data with structure invariants alone is nearly as successful as
the combined use of two separate methods, assigning values to
13 of the 16 reflections-i.e., the electrostatic potential map
closely resembles the one calculated with all data.