English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Functional neuroanatomical mapping in insects by [3H]2-deoxy-D-glucose at electron microscopical resolution

MPS-Authors
/persons/resource/persons246243

Buchner,  S
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

/persons/resource/persons246234

Buchner,  E
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Buchner, S., & Buchner, E. (1982). Functional neuroanatomical mapping in insects by [3H]2-deoxy-D-glucose at electron microscopical resolution. Neuroscience Letters, 28(3), 235-240. doi:10.1016/0304-3940(82)90063-5.


Cite as: http://hdl.handle.net/21.11116/0000-0006-67B9-6
Abstract
Electron microscope autoradiography of insect nervous tissue prepared for ‘activity staining’ by the deoxyglucose method provides information on metabolic activity of small identifiable nerve fibers. Grain counts over two sets of axon profiles selected for high and low radioactivity, respectively, indicate that the dynamic range of the present method comprises about one order of magnitude. It is demonstrated that, although a small fraction of the radioactive label is lost during flotation of the thin sections on a water surface, the distribution of the remaining label is not appreciably altered and reflects stimulus-induced nervous activity.