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Journal Article

A piezoelectric device to aid penetration of small nerve fibers with microelectrodes

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Hengstenberg,  R
Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Hengstenberg, R. (1981). A piezoelectric device to aid penetration of small nerve fibers with microelectrodes. Journal of Neuroscience Methods, 4(3), 249-255. doi:10.1016/0165-0270(81)90036-4.


Cite as: http://hdl.handle.net/21.11116/0000-0006-6C3B-0
Abstract
A small piezoelectric device for cell penetration is described. It retracts the micropipette slowly by electrostriction, and pushes it very fast (< 5 μsec) forward by short-circuiting the transducer. The design, operation circuit, and performance under test conditions are described. Penetration examples from small nerve fibers (< 5 μm) show that membrane puncture occurs only with the fast forward push. Cells are not noticeably damaged, even if the device is repeatedly operated after cell penetration.