Mono-, di- and trimeric PS I reaction center complexes isolated from the 
thermophilic cyanobacterium Synechococcus sp.: Size, shape and activity

Rögner, Matthias; Mühlenhoff, Ulrich; Boekema, Egbert J.; Witt, Horst Tobias

doi:10.1016/0005-2728(90)90074-E

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Mono-, di- and trimeric PS I reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus sp.: Size, shape and activity

MPS-Authors

/persons/resource/persons247340

Boekema, Egbert J.
Fritz Haber Institute, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Rögner, M., Mühlenhoff, U., Boekema, E. J., & Witt, H. T. (1990). Mono-, di- and trimeric PS I reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus sp.: Size, shape and activity. Biochimica et Biophysica Acta, Bioenergetics, 1015(3), 415-424. doi:10.1016/0005-2728(90)90074-E.

Cite as: https://hdl.handle.net/21.11116/0000-0006-705B-6

Abstract

Photosystem I preparations from the cyanobacterium Synechococcus sp. were treated with high concentrations of Tris and octyl glucoside at alkaline pH and elevated temperature. A sucrose density gradient yielded three pigment-protein complexes; these were further purified on a HPLC anion-exchange column. In contrast to SDS-PAGE under non-denaturing conditions, this method produces homogeneous, highly active PS I particles in large quantities using mild solubilization conditions. Gel-filtration HPLC, SDS-PAGE, antenna ChlChla₁ ratio and electron microscopy images suggest that the three complexes represent monomeric, dimeric and trimeric forms of a minimal reaction center I unit. The size of this monomeric complex is 15.3 × 10.6 × 6.4 nm (length × width × height) as determined by electron microscopy. The apparent molecular mass - including 65 antenna chlorophylls per Chl a₁, but excluding the detergent shell - is estimated by three different methods to be (235 ± 25) kDa.