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Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

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Frosz,  Michael H.
Fibre Fabrication and Glass Studio, Technology Development and Service Units, Max Planck Institute for the Science of Light, Max Planck Society;
Russell Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Zeltner,  Richard
Russell Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Russell,  Philip St. J.
Russell Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Citation

Klioutchnikov, A., Wallace, D. J., Frosz, M. H., Zeltner, R., Sawinski, J., Pawlak, V., et al. (2020). Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats. Nature methods, 17, 509-513. doi:10.1038/s41592-020-0817-9.


Cite as: https://hdl.handle.net/21.11116/0000-0006-6CED-7
Abstract
We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 euronal activity for >1 h in freely moving rats performing a range of behaviors.