Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase 
contrast microscopy

Faizi, Hammad A.; Reeves, Cody J.; Georgiev, Vasil; Vlahovska, Petia M.; Dimova, Rumiana

doi:10.1039/D0SM00943A

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Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

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Georgiev, Vasil
Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Dimova, Rumiana
Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Faizi, H. A., Reeves, C. J., Georgiev, V., Vlahovska, P. M., & Dimova, R. (2020). Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy. Soft Matter, 16(39), 8996-9001. doi:10.1039/D0SM00943A.

Cite as: https://hdl.handle.net/21.11116/0000-0006-769D-5

Abstract

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.