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Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography

MPS-Authors
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Grünbein,  Marie Luise
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society;

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Stricker,  Miriam
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Nass Kovács,  Gabriela
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;

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Kloos,  Marco
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Doak,  Bruce
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;

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Shoeman,  Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Reinstein,  Jochen
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;
Molecular chaperones, Max Planck Institute for Medical Research, Max Planck Society;

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Schlichting,  Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Heme and Flavin Enzymes, Max Planck Institute for Medical Research, Max Planck Society;
Photoreceptors, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Grünbein, M. L., Stricker, M., Nass Kovács, G., Kloos, M., Doak, B., Shoeman, R. L., et al. (2020). Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography. Nature methods, e-pup(47-3), 1-9. doi:10.1038/s41592-020-0847-3.


Cite as: http://hdl.handle.net/21.11116/0000-0006-7007-4
Abstract
Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.