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Journal Article

FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing.

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Lemaitre,  Regis P.
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Bogdanova,  Aliona
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Borgonovo,  Barbara
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Woodruff,  Jeffrey
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Drechsel,  David N.
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Lemaitre, R. P., Bogdanova, A., Borgonovo, B., Woodruff, J., & Drechsel, D. N. (2019). FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing. BMC biotechnology, 19(1): 20. doi:10.1186/s12896-019-0512-z.


Cite as: https://hdl.handle.net/21.11116/0000-0006-7DA8-1
Abstract
Baculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time-consuming, offer limited options for protein tagging, and are unsuitable for secreted proteins requiring proteolytic maturation, such as TGF-β family growth factors.