Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Genetic Modification of Brain Organoids.


Heide,  Michael
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;


Huttner,  Wieland
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Fischer, J., Heide, M., & Huttner, W. (2019). Genetic Modification of Brain Organoids. Frontiers in cellular neuroscience, 13: 558. doi:10.3389/fncel.2019.00558.

Cite as: https://hdl.handle.net/21.11116/0000-0006-7DB4-3
Brain organoids have become increasingly used systems allowing 3D-modeling of human brain development, evolution, and disease. To be able to make full use of these modeling systems, researchers have developed a growing toolkit of genetic modification techniques. These techniques can be applied to mature brain organoids or to the preceding embryoid bodies (EBs) and founding cells. This review will describe techniques used for transient and stable genetic modification of brain organoids and discuss their current use and respective advantages and disadvantages. Transient approaches include adeno-associated virus (AAV) and electroporation-based techniques, whereas stable genetic modification approaches make use of lentivirus (including viral stamping), transposon and CRISPR/Cas9 systems. Finally, an outlook as to likely future developments and applications regarding genetic modifications of brain organoids will be presented.