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Journal Article

A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

MPS-Authors
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Maricic,  Tomislav
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Ayinuer-Petri,  Aximu
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Essel,  Elena
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Gansauge,  Marie-Theres
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Kanis,  Philipp
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Macak,  Dominik
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Riesenberg,  Stephan
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Bokelmann,  Lukas
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Zeberg,  Hugo
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Meyer,  Matthias
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Pääbo,  Svante
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society;

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Fulltext (public)

Maricic_A-direct_PLoSOne_2020.pdf
(Publisher version), 2MB

Supplementary Material (public)
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Citation

Maricic, T., Nickel, O., Ayinuer-Petri, A., Essel, E., Gansauge, M.-T., Kanis, P., et al. (2020). A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2. PLoS One, 15(12): e0244824. doi:10.1371/journal.pone.0244824.


Cite as: http://hdl.handle.net/21.11116/0000-0006-9D73-8
Abstract
SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast approach to detect SARSCoV-2 in single or pooled gargle lavages (“mouthwashes”). With this approach, we test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches could be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.