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A Short Linear Motif in Scaffold Nup145C Connects Y-complex With Pre-Assembled Outer Ring Nup82 Complex

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Teimer, R., Kosinski, J., von Appen, A., Beck, M., & Hurt, E. (2017). A Short Linear Motif in Scaffold Nup145C Connects Y-complex With Pre-Assembled Outer Ring Nup82 Complex. Nature Communications, 8: 1107. doi:10.1038/s41467-017-01160-9.


Cite as: http://hdl.handle.net/21.11116/0000-0006-A1A4-A
Abstract
Nucleocytoplasmic transport occurs through nuclear pore complexes (NPCs), which are formed from multiple copies of ~30 different nucleoporins (Nups) and inserted into the double nuclear membrane. Many of these Nups are organized into subcomplexes, of which the Y-shaped Nup84 complex is the major constituent of the nuclear and cytoplasmic rings. The Nup82-Nup159-Nsp1 complex is another module that, however, is only assembled into the cytoplasmic ring. By means of crosslinking mass spectrometry, biochemical reconstitution, and molecular modeling, we identified a short linear motif in the unstructured N-terminal region of Chaetomium thermophilum Nup145C, a subunit of the Y-complex, that is sufficient to recruit the Nup82 complex, but only in its assembled state. This finding points to a more general mechanism that short linear motifs in structural Nups can act as sensors to cooperatively connect pre-assembled NPC modules, thereby facilitating the formation and regulation of the higher-order NPC assembly.