Purification of escherichia coli DNA helicase I from plasmid-transformed cells

Benz, I.; Müller, H.; Abdel-Monem, Mahmoud; Hoffman-Berling, Hartmut

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Purification of escherichia coli DNA helicase I from plasmid-transformed cells

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Benz, I.
Max Planck Institute for Medical Research, Max Planck Society;

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Abdel-Monem, Mahmoud
Max Planck Institute for Medical Research, Max Planck Society;

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Hoffman-Berling, Hartmut
Max Planck Institute for Medical Research, Max Planck Society;

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Benz, I., Müller, H., Abdel-Monem, M., & Hoffman-Berling, H. (1986). Purification of escherichia coli DNA helicase I from plasmid-transformed cells. Acta Microbiologica Polonica, 35, 191-198. Retrieved from https://pubmed.ncbi.nlm.nih.gov/2436446/.

Cite as: https://hdl.handle.net/21.11116/0000-0006-A1FB-9

Abstract

DNA helicase I was purified in large quantity from Escherichia coli cells harboring a plasmid that carries the gene encoding helicase I--the traI gene of the F sex factor--cloned in a high copy number vector. Electron microscopic studies on the purified material reveal new properties of the enzyme protein.