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Toward the atomic structure of the nuclear pore complex: when top down meets bottom up

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Hoelz, A., Glavy, J. S., & Beck, M. (2016). Toward the atomic structure of the nuclear pore complex: when top down meets bottom up. Nature Structural and Molecular Biology, 23(7), 624-630. doi:10.1038/nsmb.3244.


Cite as: http://hdl.handle.net/21.11116/0000-0006-A844-0
Abstract
Elucidating the structure of the nuclear pore complex (NPC) is a prerequisite for understanding the molecular mechanism of nucleocytoplasmic transport. However, owing to its sheer size and flexibility, the NPC is unapproachable by classical structure determination techniques and requires a joint effort of complementary methods. Whereas bottom-up approaches rely on biochemical interaction studies and crystal-structure determination of NPC components, top-down approaches attempt to determine the structure of the intact NPC in situ. Recently, both approaches have converged, thereby bridging the resolution gap from the higher-order scaffold structure to near-atomic resolution and opening the door for structure-guided experimental interrogations of NPC function.