Toward the atomic structure of the nuclear pore complex: when top down meets 
bottom up

Hoelz, André; Glavy, Joseph S.; Beck, Martin

doi:10.1038/nsmb.3244

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Toward the atomic structure of the nuclear pore complex: when top down meets bottom up

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Hoelz, A., Glavy, J. S., & Beck, M. (2016). Toward the atomic structure of the nuclear pore complex: when top down meets bottom up. Nature Structural and Molecular Biology, 23(7), 624-630. doi:10.1038/nsmb.3244.

Cite as: https://hdl.handle.net/21.11116/0000-0006-A844-0

Abstract

Elucidating the structure of the nuclear pore complex (NPC) is a prerequisite for understanding the molecular mechanism of nucleocytoplasmic transport. However, owing to its sheer size and flexibility, the NPC is unapproachable by classical structure determination techniques and requires a joint effort of complementary methods. Whereas bottom-up approaches rely on biochemical interaction studies and crystal-structure determination of NPC components, top-down approaches attempt to determine the structure of the intact NPC in situ. Recently, both approaches have converged, thereby bridging the resolution gap from the higher-order scaffold structure to near-atomic resolution and opening the door for structure-guided experimental interrogations of NPC function.