Structure Determination of the Nuclear Pore Complex With Three-Dimensional Cryo 
Electron Microscopy

von Appen, Alexander; Beck, Martin

doi:10.1016/j.jmb.2016.01.004

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Structure Determination of the Nuclear Pore Complex With Three-Dimensional Cryo Electron Microscopy

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von Appen, A., & Beck, M. (2016). Structure Determination of the Nuclear Pore Complex With Three-Dimensional Cryo Electron Microscopy. Journal of Molecular Biology (London), 428(10 Pt A), 2001-2010. doi:10.1016/j.jmb.2016.01.004.

Cite as: https://hdl.handle.net/21.11116/0000-0006-A862-E

Abstract

Determining the structure of the nuclear pore complex (NPC) imposes an enormous challenge due to its size, intricate composition and membrane-embedded nature. In vertebrates, about 1000 protein building blocks assemble into a 110-MDa complex that fuses the inner and outer membranes of a cell's nucleus. Here, we review the recent progress in understanding the in situ architecture of the NPC with a specific focus on approaches using three-dimensional cryo electron microscopy. We discuss technological benefits and limitations and give an outlook toward obtaining a high-resolution structure of the NPC.