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Genomic characterization of filamentous phage vB_VpaI_VP-3218, an inducible prophage of Vibrio parahaemolyticus.

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Garin-Fernandez,  Alexa
Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Garin-Fernandez, A., Glöckner, F. O., & Wichels, A. (2020). Genomic characterization of filamentous phage vB_VpaI_VP-3218, an inducible prophage of Vibrio parahaemolyticus. Marine Genomics, 100767-100767. doi:10.1016/j.margen.2020.100767.


Cite as: https://hdl.handle.net/21.11116/0000-0006-B761-E
Abstract
The seawater temperature rise can promote the growth of potentially
pathogenic Vibrio species. In the North Sea, V. parahaemolyticus strains
have been isolated and characterized. These strains contain prophages
that may contribute to the emergence of pathogenic strains in the marine
environment. Here, we present the genome structure and possible
biological functions of the inducible phage vB_VpaI_VP-3218, a novel
filamentous phage carried by the V. parahaemolyticus strain VN-3218.
Prophages of the strain VN-3218 were induced with mitomycin C and the
DNA from the phage induction was sequenced. Two incomplete prophages
were identified, only one complete phage genome with length of 11,082bp
was characterized. The phage vB_VpaI_VP-3218 belongs to the Inoviridae
family and shows close homology to the Saetivirus genus. This phage can
integrate into the chromosomal host genome and carries host-related
regions absent in similar phage genomes, suggesting that this phage
might integrate in other Vibrio host genomes from the environment.
Furthermore, this phage might have a role in pathogenicity due to
potential zonula occludens toxin genes. Based on its genomic similarity,
the genome of vB_VpaI_VP-3218 phage probably integrates into the
lysogen's chromosome and replicates as episome. This study complements
prophage induction and bioinformatic studies applied to non-model
species of potentially pathogenic Vibrio species. The characterization
of this phage provides new insights with respect to the presence of
filamentous phages in environmental V. parahaemolyticus strains, which
might have a role in the emergence of new pathogenic strains in the
North Sea.