Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT

Strauss, Sebastian; Jungmann, Ralf

doi:10.1038/s41592-020-0869-x

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT

MPS-Authors

/persons/resource/persons208765

Strauss, Sebastian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172959

Jungmann, Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Strauss, S., & Jungmann, R. (2020). Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT. NATURE METHODS, 17, 789-791. doi:10.1038/s41592-020-0869-x.

引用: https://hdl.handle.net/21.11116/0000-0006-BA3F-3

要旨

DNA-PAINT's imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed limit and is only applicable to imaging of single targets. To further improve acquisition speed, we introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold-faster sampling in comparison to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.
Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.