Three-dimensional crystals of a membrane protein complex: The photosynthetic 
reaction centre from Rhodopseudomonas viridis

Michel, Hartmut

doi:10.1016/0022-2836(82)90216-9

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Three-dimensional crystals of a membrane protein complex: The photosynthetic reaction centre from Rhodopseudomonas viridis

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Michel, Hartmut
Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Michel, H. (1982). Three-dimensional crystals of a membrane protein complex: The photosynthetic reaction centre from Rhodopseudomonas viridis. Journal of Molecular Biology (London), 158(3), 567-572. doi:10.1016/0022-2836(82)90216-9.

Cite as: https://hdl.handle.net/21.11116/0000-0006-C9BF-1

Abstract

Reaction centres of photosynthesis isolated from the photosynthetic bacterium Rhodopseudomonas viridis by a single step of molecular sieve chromatography were crystallized. The isolated and the crystallized reaction centres contain four different protein subunits, including a membrane-bound cytochrome. Crystallization was achieved by salt precipitation using 2 to 3 m-ammonium sulphate as a precipitating agent in the presence of N,N-dimethyldodecylamine N-oxide as detergent and heptane-1,2,3-triol. Large tetragonal crystals of space group P4₁2₁2 or its enantiomorph diffracting to beyond 2.5 Å were obtained.