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High-precision protein-tracking with interferometric scattering microscopy

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Taylor,  Richard W.
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

Holler ,  Cornelia
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Gholami Mahmoodabadi,  Reza
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

Küppers ,  Michelle
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

Mirzaalian Dastjerdi,  Houman
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Zaburdaev,  Vasily
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

Schambony,  Alexandra
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Sandoghdar,  Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

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Citation

Taylor, R. W., Holler, C., Gholami Mahmoodabadi, R., Küppers, M., Mirzaalian Dastjerdi, H., Zaburdaev, V., et al. (2020). High-precision protein-tracking with interferometric scattering microscopy. Frontiers in Cell and Developmental Biology, 8: 590158. doi:10.3389/fcell.2020.590158.


Cite as: https://hdl.handle.net/21.11116/0000-0006-CD81-1
Abstract
The mobility of proteins and lipids within the cell, sculpted oftentimes by the organisation of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behaviour. Interferometric scattering (iSCAT) microscopy is an emerging technique well suited for visualising the diffusion of gold nanoparticle-labelled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualising the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.