Environmentally sensitive color‐shifting fluorophores for bioimaging

Wang, Lu; Hiblot, Julien; Popp, Christoph; Xue, Lin; Johnsson, Kai

doi:10.1002/anie.202008357

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Environmentally sensitive color‐shifting fluorophores for bioimaging

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Wang, Lu
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212621

Hiblot, Julien
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons249804

Popp, Christoph
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212625

Xue, Lin
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203696

Johnsson, Kai
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

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https://onlinelibrary.wiley.com/doi/epdf/10.1002/anie.202008357
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Citation

Wang, L., Hiblot, J., Popp, C., Xue, L., & Johnsson, K. (2020). Environmentally sensitive color‐shifting fluorophores for bioimaging. Angewandte Chemie International Edition, 59(49), 21880-21884. doi:10.1002/anie.202008357.

Cite as: http://hdl.handle.net/21.11116/0000-0006-D628-C

Abstract

We introduce color‐shifting fluorophores which reversibly switch between a green and red fluorescent form via an intramolecular spirocyclization. The equilibrium of spirocyclization is environmentally sensitive and can be directly measured by determining the ratio of red to green fluorescence, allowing the generation of ratiometric fluorescent probes and biosensors. Specifically, we develop a ratiometric biosensor for imaging calcium ions (Ca 2+ ) in living cells, ratiometric probes for different proteins and a bioassay for the quantification of nicotinamide adenine dinucleotide phosphate.